《植物生理学报》 2014, 50(11): 1651-1658

水稻OsWRKY转录因子对非生物胁迫响应的重叠表达特性分析

孙利军¹, 黄磊², 李大勇², 张慧娟², 宋凤鸣^2,*

¹南通大学公共卫生学院生命分析化学研究所, 江苏南通226019; ²浙江大学生物技术研究所水稻生物学国家重点实验室, 杭州310029

通信作者：宋凤鸣；E-mail: fmsong@zju.edu.cn；Tel: 0571-88982481

摘　要：

WRKY转录因子是植物一类比较大的基因家族, 在水稻中已鉴定出102个成员。研究表明WRKY转录因子在植物生长发育、抗病耐逆等方面都具有重要的作用。本研究利用基因芯片数据结合实时定量分析, 对水稻OsWRKY转录因子基因在不同的非生物逆境下的表达进行了分析, 发现至少有33个OsWRKY基因同时对任何两种非生物胁迫因子做出响应, 且所选20个基因中, 13个基因可被ABA所诱导。OsWRKY基因这种重叠表达的特性, 预示着这些基因在非生物逆境中具有功能多效性, 对于培育抗逆境水稻品种具有重要的理论与实践意义。

关键词：水稻; OsWRKY转录因子; 非生物胁迫响应; 重叠表达

收稿：2014-06-19   修定：2014-09-03

资助：国家转基因重大专项(2011ZX08009-003-001)、国家自然科学基金项目(30971880和31400315)、教育部高校博士点专项基金(20120101110070)、江苏省自然科学青年基金(BK20130389)、南通大学预研项目(13ZY012)、南通大学博士引进人才启动基金(13R41)和南通大学研究生科技创新计划项目(YKC13043)。

Comprehensive Expression Analysis Suggests Overlapping of Rice OsWRKY Transcription Factor Genes during Abiotic Stress Responses

SUN Li-Jun¹, HUANG Lei², LI Da-Yong², ZHANG Hui-Juan², SONG Feng-Ming^2,*

¹Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong, Jiangsu 226019, China; ²State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding author: SONG Feng-Ming; E-mail: fmsong@zju.edu.cn; Tel: 0571-88982481

Abstract:

WRKY transcription factors as one large and plant-specific gene family have been identified 102 members in rice. Extensive studies have revealed that WRKY transcription factors not only play important roles in plant growth and development, but also have functions in regulation of responses to biotic and abiotic stresses. In the present study, microarray data analyses revealed that about 33 OsWRKY genes were overlapping expressed during in various abiotic (salinity, drought, cold, and hot) conditions, 13 among 20 representative Os-WRKY genes were up-regulated in response to ABA. The results of this study provided not only an evidence for the roles of OsWRKY genes associated with crosstalk among abiotic stresses and ABA response pathway, but also a useful reference for the functional analysis of OsWRKY genes in stress tolerance.

Key words: rice; OsWRKY transcription factors; abiotic stress responses; overlapping expression